c3ar antagonist Search Results


sb290157  (MedChemExpress)
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MedChemExpress sb290157
Sb290157, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
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c3a receptor antagonist sb290157  (Cayman Chemical)
90
Cayman Chemical c3a receptor antagonist sb290157
Glomerular and tubular C3 and <t>C3aR</t> staining in Stx2/LPS mice: ( A ) Representative images and quantification of intraglomerular and tubular C3 staining (green) in control and Stx2/LPS-injected mice at 48 h; ( B ) Representative images and quantification of intraglomerular and tubular C3aR expression (red) in control and Stx2/LPS-injected mice. Scale bars: 20 μm. Results are presented as mean ± SEM (control n = 4, Stx2/LPS n = 7), and unpaired Student’s t test was used. * p < 0.05, ** p < 0.01.
C3a Receptor Antagonist Sb290157, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/c3a receptor antagonist sb290157/product/Cayman Chemical
Average 90 stars, based on 1 article reviews
c3a receptor antagonist sb290157 - by Bioz Stars, 2026-02
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c3ar antagonist sb 290157  (SmithKline Corporation)
90
SmithKline Corporation c3ar antagonist sb 290157
Glomerular and tubular C3 and <t>C3aR</t> staining in Stx2/LPS mice: ( A ) Representative images and quantification of intraglomerular and tubular C3 staining (green) in control and Stx2/LPS-injected mice at 48 h; ( B ) Representative images and quantification of intraglomerular and tubular C3aR expression (red) in control and Stx2/LPS-injected mice. Scale bars: 20 μm. Results are presented as mean ± SEM (control n = 4, Stx2/LPS n = 7), and unpaired Student’s t test was used. * p < 0.05, ** p < 0.01.
C3ar Antagonist Sb 290157, supplied by SmithKline Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/c3ar antagonist sb 290157/product/SmithKline Corporation
Average 90 stars, based on 1 article reviews
c3ar antagonist sb 290157 - by Bioz Stars, 2026-02
90/100 stars
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Image Search Results


Glomerular and tubular C3 and C3aR staining in Stx2/LPS mice: ( A ) Representative images and quantification of intraglomerular and tubular C3 staining (green) in control and Stx2/LPS-injected mice at 48 h; ( B ) Representative images and quantification of intraglomerular and tubular C3aR expression (red) in control and Stx2/LPS-injected mice. Scale bars: 20 μm. Results are presented as mean ± SEM (control n = 4, Stx2/LPS n = 7), and unpaired Student’s t test was used. * p < 0.05, ** p < 0.01.

Journal: Cells

Article Title: Shiga Toxin 2 Triggers C3a-Dependent Glomerular and Tubular Injury through Mitochondrial Dysfunction in Hemolytic Uremic Syndrome

doi: 10.3390/cells11111755

Figure Lengend Snippet: Glomerular and tubular C3 and C3aR staining in Stx2/LPS mice: ( A ) Representative images and quantification of intraglomerular and tubular C3 staining (green) in control and Stx2/LPS-injected mice at 48 h; ( B ) Representative images and quantification of intraglomerular and tubular C3aR expression (red) in control and Stx2/LPS-injected mice. Scale bars: 20 μm. Results are presented as mean ± SEM (control n = 4, Stx2/LPS n = 7), and unpaired Student’s t test was used. * p < 0.05, ** p < 0.01.

Article Snippet: Animals were randomly allocated to the following groups: group 1 ( n = 7), C57BL/6 mice given purified Stx2 (120 ng per mouse) plus LPS (60 μg per mouse; L2630, Merck, Darmstadt, Germany) by intraperitoneal injection to induce HUS; group 2 ( n = 7), Stx2/LPS-treated mice given the C3a receptor antagonist SB290157 (Cayman Chemical, Ann Arbor, MI, USA) at a dose of 2.5 mg/kg [ ], by intraperitoneal injection, 1 h before Stx2/LPS injection and twice a day for two days.

Techniques: Staining, Control, Injection, Expressing

Treatment with the C3aR antagonist limits glomerular mitochondrial alterations in Stx2/LPS mice: ( A ) Representative transmission electron microscope images showing mitochondrial morphology in podocytes of control and Stx2/LPS mice, given vehicle or C3aR antagonist (C3aRa). Scale bars: 500 nm; ( B ) Quantification of mitochondrial length and area and ( C ) characterization of mitochondrial subpopulations in podocytes. Results are expressed as mean ± SEM (control: n = 516 mitochondria analyzed in n = 6 glomeruli of n = 2 mice; Stx2/LPS + vehicle: n = 411 mitochondria analyzed in n = 7 glomeruli of n = 3 mice; Stx2/LPS + C3aRa: n = 529 mitochondria analyzed in n = 7 glomeruli of n = 3 mice); ( D ) Representative images and quantification of glomerular VDAC (upper panels) and ATP5I (bottom panels) staining in controls and Stx2/LPS mice given vehicle or C3aRa. Podocytes are indicated by red arrowheads. Scale bars: 20 μm. Results are expressed as mean ± SEM (VDAC: control n = 4, Stx2/LPS + vehicle n = 5, Stx2/LPS + C3aRa n = 4; ATP5I: control n = 4, Stx2/LPS + vehicle n = 7, Stx2/LPS + C3aRa n = 7), and ANOVA with Tukey multiple-comparisons test was used. * p < 0.05, ** p < 0.01.

Journal: Cells

Article Title: Shiga Toxin 2 Triggers C3a-Dependent Glomerular and Tubular Injury through Mitochondrial Dysfunction in Hemolytic Uremic Syndrome

doi: 10.3390/cells11111755

Figure Lengend Snippet: Treatment with the C3aR antagonist limits glomerular mitochondrial alterations in Stx2/LPS mice: ( A ) Representative transmission electron microscope images showing mitochondrial morphology in podocytes of control and Stx2/LPS mice, given vehicle or C3aR antagonist (C3aRa). Scale bars: 500 nm; ( B ) Quantification of mitochondrial length and area and ( C ) characterization of mitochondrial subpopulations in podocytes. Results are expressed as mean ± SEM (control: n = 516 mitochondria analyzed in n = 6 glomeruli of n = 2 mice; Stx2/LPS + vehicle: n = 411 mitochondria analyzed in n = 7 glomeruli of n = 3 mice; Stx2/LPS + C3aRa: n = 529 mitochondria analyzed in n = 7 glomeruli of n = 3 mice); ( D ) Representative images and quantification of glomerular VDAC (upper panels) and ATP5I (bottom panels) staining in controls and Stx2/LPS mice given vehicle or C3aRa. Podocytes are indicated by red arrowheads. Scale bars: 20 μm. Results are expressed as mean ± SEM (VDAC: control n = 4, Stx2/LPS + vehicle n = 5, Stx2/LPS + C3aRa n = 4; ATP5I: control n = 4, Stx2/LPS + vehicle n = 7, Stx2/LPS + C3aRa n = 7), and ANOVA with Tukey multiple-comparisons test was used. * p < 0.05, ** p < 0.01.

Article Snippet: Animals were randomly allocated to the following groups: group 1 ( n = 7), C57BL/6 mice given purified Stx2 (120 ng per mouse) plus LPS (60 μg per mouse; L2630, Merck, Darmstadt, Germany) by intraperitoneal injection to induce HUS; group 2 ( n = 7), Stx2/LPS-treated mice given the C3a receptor antagonist SB290157 (Cayman Chemical, Ann Arbor, MI, USA) at a dose of 2.5 mg/kg [ ], by intraperitoneal injection, 1 h before Stx2/LPS injection and twice a day for two days.

Techniques: Transmission Assay, Microscopy, Control, Staining

The C3aR blockade reduced proximal tubular cell apoptosis and restored megalin expression in Stx2/LPS mice. ( A , B ) Representative images and quantification of ( A ) cleaved caspase-3 (red) and ( B ) megalin (red) in control and Stx2/LPS mice given vehicle or C3aRa. Arrowheads indicate loss of megalin expression on the proximal tubular cell brush border. Cell membranes and nuclei were stained with FITC-WGA-lectin (green) and DAPI (blue), respectively. Scale bars: 20 μm. Results are expressed as mean ± SEM ( n = 4 per each group), and ANOVA with Tukey multiple-comparisons test was used. * p < 0.05, ** p < 0.01.

Journal: Cells

Article Title: Shiga Toxin 2 Triggers C3a-Dependent Glomerular and Tubular Injury through Mitochondrial Dysfunction in Hemolytic Uremic Syndrome

doi: 10.3390/cells11111755

Figure Lengend Snippet: The C3aR blockade reduced proximal tubular cell apoptosis and restored megalin expression in Stx2/LPS mice. ( A , B ) Representative images and quantification of ( A ) cleaved caspase-3 (red) and ( B ) megalin (red) in control and Stx2/LPS mice given vehicle or C3aRa. Arrowheads indicate loss of megalin expression on the proximal tubular cell brush border. Cell membranes and nuclei were stained with FITC-WGA-lectin (green) and DAPI (blue), respectively. Scale bars: 20 μm. Results are expressed as mean ± SEM ( n = 4 per each group), and ANOVA with Tukey multiple-comparisons test was used. * p < 0.05, ** p < 0.01.

Article Snippet: Animals were randomly allocated to the following groups: group 1 ( n = 7), C57BL/6 mice given purified Stx2 (120 ng per mouse) plus LPS (60 μg per mouse; L2630, Merck, Darmstadt, Germany) by intraperitoneal injection to induce HUS; group 2 ( n = 7), Stx2/LPS-treated mice given the C3a receptor antagonist SB290157 (Cayman Chemical, Ann Arbor, MI, USA) at a dose of 2.5 mg/kg [ ], by intraperitoneal injection, 1 h before Stx2/LPS injection and twice a day for two days.

Techniques: Expressing, Control, Staining

The C3aR blockade restores mitochondrial morphology and function in proximal tubular cells in Stx2/LPS mice. ( A , B ) Representative transmission electron microscope images and morphometric analysis showing mitochondrial morphology in proximal tubular cells of control and Stx2/LPS mice, given vehicle or C3aRa. Scale bars: 500 nm. Results are expressed as mean ± SEM (control: n = 6768 mitochondria analyzed in n = 3 mice; Stx2/LPS + vehicle: n = 4238 mitochondria analyzed in n = 3 mice; Stx2/LPS + C3aRa: n = 7100 mitochondria analyzed in n = 3 mice). ( C ) Representative images and quantification of tubular VDAC (upper panels) and ATP5I (bottom panels) stainings in control and Stx2/LPS mice with or without C3aRa. Scale bars: 20 μm. Results are expressed as mean ± SEM (VDAC: control n = 4, Stx2/LPS + vehicle n = 5, Stx2/LPS + C3aRa n = 4; ATP5I: control n = 4, Stx2/LPS + vehicle n = 7, Stx2/LPS + C3aRa n = 7). ANOVA with Tukey multiple-comparisons test was used. * p < 0.05, ** p < 0.01.

Journal: Cells

Article Title: Shiga Toxin 2 Triggers C3a-Dependent Glomerular and Tubular Injury through Mitochondrial Dysfunction in Hemolytic Uremic Syndrome

doi: 10.3390/cells11111755

Figure Lengend Snippet: The C3aR blockade restores mitochondrial morphology and function in proximal tubular cells in Stx2/LPS mice. ( A , B ) Representative transmission electron microscope images and morphometric analysis showing mitochondrial morphology in proximal tubular cells of control and Stx2/LPS mice, given vehicle or C3aRa. Scale bars: 500 nm. Results are expressed as mean ± SEM (control: n = 6768 mitochondria analyzed in n = 3 mice; Stx2/LPS + vehicle: n = 4238 mitochondria analyzed in n = 3 mice; Stx2/LPS + C3aRa: n = 7100 mitochondria analyzed in n = 3 mice). ( C ) Representative images and quantification of tubular VDAC (upper panels) and ATP5I (bottom panels) stainings in control and Stx2/LPS mice with or without C3aRa. Scale bars: 20 μm. Results are expressed as mean ± SEM (VDAC: control n = 4, Stx2/LPS + vehicle n = 5, Stx2/LPS + C3aRa n = 4; ATP5I: control n = 4, Stx2/LPS + vehicle n = 7, Stx2/LPS + C3aRa n = 7). ANOVA with Tukey multiple-comparisons test was used. * p < 0.05, ** p < 0.01.

Article Snippet: Animals were randomly allocated to the following groups: group 1 ( n = 7), C57BL/6 mice given purified Stx2 (120 ng per mouse) plus LPS (60 μg per mouse; L2630, Merck, Darmstadt, Germany) by intraperitoneal injection to induce HUS; group 2 ( n = 7), Stx2/LPS-treated mice given the C3a receptor antagonist SB290157 (Cayman Chemical, Ann Arbor, MI, USA) at a dose of 2.5 mg/kg [ ], by intraperitoneal injection, 1 h before Stx2/LPS injection and twice a day for two days.

Techniques: Transmission Assay, Microscopy, Control

Treatment with the C3aR antagonist limits tubulin alterations in proximal tubules of Stx2/LPS mice. Representative images of tubulin expression (green) in control and Stx2/LPS mice receiving vehicle or C3aRa. Nuclei were stained with DAPI (blue). Scale bars: 20 μm.

Journal: Cells

Article Title: Shiga Toxin 2 Triggers C3a-Dependent Glomerular and Tubular Injury through Mitochondrial Dysfunction in Hemolytic Uremic Syndrome

doi: 10.3390/cells11111755

Figure Lengend Snippet: Treatment with the C3aR antagonist limits tubulin alterations in proximal tubules of Stx2/LPS mice. Representative images of tubulin expression (green) in control and Stx2/LPS mice receiving vehicle or C3aRa. Nuclei were stained with DAPI (blue). Scale bars: 20 μm.

Article Snippet: Animals were randomly allocated to the following groups: group 1 ( n = 7), C57BL/6 mice given purified Stx2 (120 ng per mouse) plus LPS (60 μg per mouse; L2630, Merck, Darmstadt, Germany) by intraperitoneal injection to induce HUS; group 2 ( n = 7), Stx2/LPS-treated mice given the C3a receptor antagonist SB290157 (Cayman Chemical, Ann Arbor, MI, USA) at a dose of 2.5 mg/kg [ ], by intraperitoneal injection, 1 h before Stx2/LPS injection and twice a day for two days.

Techniques: Expressing, Control, Staining

Stx2 increases podocyte and tubular cell sensitivity to C3a through the upregulation of C3aR expression in vitro. ( A ) Representative images and quantification of C3aR staining (red) in podocytes and RPTECs exposed to control medium or Stx2 (50 pM) for 15 h. Nuclei were counterstained with DAPI (blue). Scale bars: 20 μm. Results are presented as mean ± SEM (number of biological samples: n = 3 control and Stx2-treated podocytes; n = 4 control RPTECs and n = 6 Stx2-treated RPTECs), and unpaired Student’s t test was used. ( B ) Representative images of mitochondria labeled with MitoTracker in live podocytes and RPTECs incubated with control medium, C3a (1 μM, 6 h) alone, or with Stx2 (50 pM, 24 h) in the presence of C3a, added in the last 6 h. Nuclei were counterstained with Hoechst (blue). Arrowhead indicates mitochondrial swelling. The percentage of cells with an altered mitochondrial pattern, in terms of fragmentation and perinuclear redistribution, on total cells per field was quantified. Scale bars: 20 μm. Results are expressed as mean ± SEM (number of biological samples: n = 3 control, C3a and Stx2 + C3a-treated podocytes and RPTECs), and ANOVA with Tukey multiple-comparisons test was used. ** p < 0.01.

Journal: Cells

Article Title: Shiga Toxin 2 Triggers C3a-Dependent Glomerular and Tubular Injury through Mitochondrial Dysfunction in Hemolytic Uremic Syndrome

doi: 10.3390/cells11111755

Figure Lengend Snippet: Stx2 increases podocyte and tubular cell sensitivity to C3a through the upregulation of C3aR expression in vitro. ( A ) Representative images and quantification of C3aR staining (red) in podocytes and RPTECs exposed to control medium or Stx2 (50 pM) for 15 h. Nuclei were counterstained with DAPI (blue). Scale bars: 20 μm. Results are presented as mean ± SEM (number of biological samples: n = 3 control and Stx2-treated podocytes; n = 4 control RPTECs and n = 6 Stx2-treated RPTECs), and unpaired Student’s t test was used. ( B ) Representative images of mitochondria labeled with MitoTracker in live podocytes and RPTECs incubated with control medium, C3a (1 μM, 6 h) alone, or with Stx2 (50 pM, 24 h) in the presence of C3a, added in the last 6 h. Nuclei were counterstained with Hoechst (blue). Arrowhead indicates mitochondrial swelling. The percentage of cells with an altered mitochondrial pattern, in terms of fragmentation and perinuclear redistribution, on total cells per field was quantified. Scale bars: 20 μm. Results are expressed as mean ± SEM (number of biological samples: n = 3 control, C3a and Stx2 + C3a-treated podocytes and RPTECs), and ANOVA with Tukey multiple-comparisons test was used. ** p < 0.01.

Article Snippet: Animals were randomly allocated to the following groups: group 1 ( n = 7), C57BL/6 mice given purified Stx2 (120 ng per mouse) plus LPS (60 μg per mouse; L2630, Merck, Darmstadt, Germany) by intraperitoneal injection to induce HUS; group 2 ( n = 7), Stx2/LPS-treated mice given the C3a receptor antagonist SB290157 (Cayman Chemical, Ann Arbor, MI, USA) at a dose of 2.5 mg/kg [ ], by intraperitoneal injection, 1 h before Stx2/LPS injection and twice a day for two days.

Techniques: Expressing, In Vitro, Staining, Control, Labeling, Incubation

C3a decreases tubulin expression and reduces mitochondrial antioxidant defense in cultured podocytes and RPTECs. Representative western blot and densitometric analysis of ( A ) tubulin and ( B ) acetylated SOD2 (SOD2 AcK68 ) and total SOD2 protein expression in protein extracts obtained from podocytes and RPTECs incubated with control medium, C3a (1 μM, 6h) alone, or with Stx2 (50 pM, 24 h) in the presence of C3a, added in the last 6 h. SOD2 acetylation was expressed as the ratio between SOD2 AcK68 and total SOD2. Results are presented as mean ± SEM (number of biological samples for tubulin: n = 3 control and n = 6 C3a-treated podocytes; n = 6 control and n = 12 C3a-treated RPTECs), (number of biological samples for SOD2: n = 3 control, C3a and Stx2+C3a-treated podocytes and RPTECs), and unpaired Student’s t test or ANOVA with Tukey multiple-comparisons test were used. * p < 0.05, ** p < 0.01.

Journal: Cells

Article Title: Shiga Toxin 2 Triggers C3a-Dependent Glomerular and Tubular Injury through Mitochondrial Dysfunction in Hemolytic Uremic Syndrome

doi: 10.3390/cells11111755

Figure Lengend Snippet: C3a decreases tubulin expression and reduces mitochondrial antioxidant defense in cultured podocytes and RPTECs. Representative western blot and densitometric analysis of ( A ) tubulin and ( B ) acetylated SOD2 (SOD2 AcK68 ) and total SOD2 protein expression in protein extracts obtained from podocytes and RPTECs incubated with control medium, C3a (1 μM, 6h) alone, or with Stx2 (50 pM, 24 h) in the presence of C3a, added in the last 6 h. SOD2 acetylation was expressed as the ratio between SOD2 AcK68 and total SOD2. Results are presented as mean ± SEM (number of biological samples for tubulin: n = 3 control and n = 6 C3a-treated podocytes; n = 6 control and n = 12 C3a-treated RPTECs), (number of biological samples for SOD2: n = 3 control, C3a and Stx2+C3a-treated podocytes and RPTECs), and unpaired Student’s t test or ANOVA with Tukey multiple-comparisons test were used. * p < 0.05, ** p < 0.01.

Article Snippet: Animals were randomly allocated to the following groups: group 1 ( n = 7), C57BL/6 mice given purified Stx2 (120 ng per mouse) plus LPS (60 μg per mouse; L2630, Merck, Darmstadt, Germany) by intraperitoneal injection to induce HUS; group 2 ( n = 7), Stx2/LPS-treated mice given the C3a receptor antagonist SB290157 (Cayman Chemical, Ann Arbor, MI, USA) at a dose of 2.5 mg/kg [ ], by intraperitoneal injection, 1 h before Stx2/LPS injection and twice a day for two days.

Techniques: Expressing, Cell Culture, Western Blot, Incubation, Control